Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p074 | (1) | WCRB2014

Novel porcine OCT4 variants identification in blastocyst and discriminative expression analysis in adult tissues

Hwang Jae Yeon , Oh Jong-Nam , Lee Dong-Kyung , Lee Chang-Kyu

Introduction: OCT4 has been known to master gene for maintaining pluripotency in mouse inner cell mass and embryonic stem cell. However, the variant of OCT4 was false-positively detected in somatic cells and this confuses the result in stem cell and embryo development research. So in this study, we aimed to identifying novel OCT4 variants in pig and examined their expression in various adult tissues.Material and method: By comparing the sequence between ...

ra0001p225 | (1) | WCRB2014

Effect of aromatase on sperm penetration and embryo cleavage rate during IVF in pig

Oh Jong-Nam , Hwang Jae Yeon , Lee Dong-Kyung , Lee Chang-Kyu

Introduction: Marker genes for evaluating sperm fertility have been developed during a few decades in various species. The CYP19A1/Cyp19a1 has been considered to one of the most common factor related to sperm quality regardless to species. In our previous report, CYP19A1 mRNA quantity was suggested to sperm fertility marker in pig. So, in this study, we examined whether CYP19A1 level and activity are related to sperm fertility to confirm importance of CYP19A1 in porci...